How to
How to make an outstanding media (agar) plate
Whether they contain TSA, MHA, LB, M9, or any other medium, making agar plates is a simple procedure. But there are a…
Author: Raphael Hans Lwesya
Raphael Hans Lwesya is a PhD researcher and science communicator focused on bacteriophages, microbiology, and bioinformatics. He is the founder of The Phage, a platform dedicated to phage research, news, protocols and job opportunities.
PROTOCOL
How to determination of the number of phage particles in a stock (phage titration)
Phage titration (determination of the number of phage particles in stock) is an essential molecular biology technique. Most of the time, it…
PROTOCOL
Protocol for Mechanical Dissociation and DNA Extraction of sample (Using QIAmp DNA Mini Kit)
This protocol was designed for microbiome analysis in sputum (although other samples can be used with slight modifications) and was adapted and…
PROTOCOL
Bacteriophage host range determination (Culture-Based Approach)
Bacteriophage host range is by definition a repertoire of bacterial species and strains that are able to support the multiplication of the…
PHAGE THERAPY
Is phage therapy a solution to Netherton Syndrome?
Netherton Syndrome (NS) is a rare genetic disorder that significantly weakens the skin’s barrier, making patients highly susceptible to chronic infections and…
AMR
Phage therapy (Bacteriophage therapy)
Soon after Alexander Fleming’s 1928 discovery of penicillin and the beginning of Western medicine’s widespread use of antibiotics in the 1940s, Fleming…
PROTOCOL
Phage Display Procedures (Protocol)
In general, there are 5 steps for phage display technology as below: Step 1: Constructing the Phage Display Library Using sophisticated recombinant…
PROTOCOL
The multiplicity of infection (MOI) of the phage explained
The Multiplicity of Infection (MOI) is a crucial metric in bacteriophage research and is defined as the ratio of the number of…
PROTOCOL
Preparation of Phage Lysates / bacteriophage enumeration (pfu/ml)
Bacteriophage enumeration is a crucial laboratory technique used to quantify the abundance of bacteriophages, viruses that infect and replicate within bacteria. This…
PROTOCOL
Determination of phage adsorption time
The determination of phage adsorption time is a critical step in the study of phages and their interactions with bacteria. This information…