Raphael Hans Lwesya is a PhD researcher and science communicator focused on bacteriophages, microbiology, and bioinformatics. He is the founder of The Phage, a platform dedicated to phage research, news, protocols and job opportunities.
This protocol was designed for microbiome analysis in sputum (although other samples can be used with slight modifications) and was adapted and…
Bacteriophage host range is by definition a repertoire of bacterial species and strains that are able to support the multiplication of the…
Netherton Syndrome (NS) is a rare genetic disorder that significantly weakens the skin’s barrier, making patients highly susceptible to chronic infections and…
Soon after Alexander Fleming’s 1928 discovery of penicillin and the beginning of Western medicine’s widespread use of antibiotics in the 1940s, Fleming…
In general, there are 5 steps for phage display technology as below: Step 1: Constructing the Phage Display Library Using sophisticated recombinant…
The Multiplicity of Infection (MOI) is a crucial metric in bacteriophage research and is defined as the ratio of the number of…
Bacteriophage enumeration is a crucial laboratory technique used to quantify the abundance of bacteriophages, viruses that infect and replicate within bacteria. This…
The determination of phage adsorption time is a critical step in the study of phages and their interactions with bacteria. This information…
Gelatin solution, 2% To prepare a 2% (w/v) solution: Add 2 g of gelatin to a total volume of 100 ml…
Tris base HCl To prepare a 1 M solution, dissolve 121.1 g of Tris base in 800 mL of H2O. Adjust…
