PROTOCOL
Preparation of SM buffer (lambda diluent)
SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That…
Author: Raphael Hans Lwesya
Raphael Hans Lwesya is a PhD researcher and science communicator focused on bacteriophages, microbiology, and bioinformatics. He is the founder of The Phage, a platform dedicated to phage research, news, protocols and job opportunities.
PROTOCOL
Preparation of Phosphate Buffered Saline (PBS)
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a water-based salt solution…
PROTOCOL
Common media used in phage work
microbiological Media List of the media used Luria broth (LB): tryptone 10 g l−1 (HiMedia, Mumbai, India), yeast extract5 g l−1 (HiMedia,…
Know your phage
The structure, packaging and ejection of bacteriophage genome
Bacteriophages consist of a protein coat that surrounds/protects their genomes; the arrangement of the genome inside this coat has been proposed by…
PHAGE RESISTANCE
Can lysogenic phages be trained to enter into the lytic cycle?
Have you ever come across the word phage training? Phages are viruses that eat up bacteria; that’s what many of us know.…
Know your phage
The adventure of bacteriophage adsorption to the bacteria and the brownian motion
How do phages adsorb to bacteria? Did you know that phage adsorption to bacterial cells involves Brownian motion? If no, then you…
Know your phage
Review: Can phages have a third life cycle?
Have you ever heard of bacteriophages (phages)? If yes, then what about Pseudolysogeny? The two words are in one way or the…
PHAGE THERAPY
Antiviral properties of bacteriophages
Bacteriophages are viruses known to attack bacteria. Their effectiveness in eradicating the most antibiotic-resistant bacteria piqued scientists’ interest in the prospect of…
Starter package
Which kind of lab is supposed to handle bacteriophages?
Bacteriophages are viruses that affect bacteria and not plants, humans,s or any other mammals; due to their specificity, it poses no harm…
Laboratory procedure
Problems of electron microscopy (TEM) for bacteriophages
Transmission electron microscopy history Transmission electron microscope Electron microscopy has always had problems with imaging and interpretation, but the rise of digital…