PROTOCOL
Preparation of FeSO4.7H2O Solution
Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm, Whatman)
Author: Raphael Hans Lwesya
Raphael Hans Lwesya is a PhD researcher and science communicator focused on bacteriophages, microbiology, and bioinformatics. He is the founder of The Phage, a platform dedicated to phage research, news, protocols and job opportunities.
PROTOCOL
Preparation of SM buffer (lambda diluent)
SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That…
PROTOCOL
Preparation of Phosphate Buffered Saline (PBS)
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a water-based salt solution…
PROTOCOL
Common media used in phage work
microbiological Media List of the media used Luria broth (LB): tryptone 10 g l−1 (HiMedia, Mumbai, India), yeast extract5 g l−1 (HiMedia,…
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The structure, packaging and ejection of bacteriophage genome
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Have you ever come across the word phage training? Phages are viruses that eat up bacteria; that’s what many of us know.…
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The adventure of bacteriophage adsorption to the bacteria and the brownian motion
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Review: Can phages have a third life cycle?
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PHAGE THERAPY
Antiviral properties of bacteriophages
Bacteriophages are viruses known to attack bacteria. Their effectiveness in eradicating the most antibiotic-resistant bacteria piqued scientists’ interest in the prospect of…
Starter package
Which kind of lab is supposed to handle bacteriophages?
Bacteriophages are viruses that affect bacteria and not plants, humans,s or any other mammals; due to their specificity, it poses no harm…