Bacteriophage Isolation from Soil and Sewage Samples
Overview This protocol outlines the isolation and purification of bacteriophages from soil or sewage samples using bacterial host strains. Samples…
Laboratory
Protocols
Versioned, peer-reviewed, and written to be followed at the bench. Each protocol lists its expected output, approximate bench time and biosafety level.
One-Step Growth Curve: Phage Latent Period and Burst Size
The one-step growth curve experiment determines the phage life cycle duration, including latent period, rise period, and burst size, by…
Bacteriophage purification by CsCl density gradient
Overview After PEG precipitation, bacteriophages can be further purified using a caesium chloride (CsCl) density gradient, which separates intact virions…
Bacteriophage precipitation using PEG (PEG/NaCl method)
Purpose Concentrate bacteriophages from a cleared lysate using PEG (polyethylene glycol) + NaCl, then resuspend as a smaller-volume, higher-titer phage…
Sewage sample processing for bacteriophage isolation
Sewage is a rich source of diverse bacteriophages, particularly coliphages that target coliform bacteria. However, its composition can vary significantly…
Prophage Induction Using Mitomycin C
Prophages are integrated viral genomes that reside within bacterial chromosomes, remaining dormant until triggered by various environmental stimuli. One of…
Lysogenic to lytic cycle: Bacteriophage Induction
Bacteriophages, particularly template phages, have developed sophisticated tactics for breaching bacterial defenses and binding to the bacterial genome. Within their…
Sewage Sample Collection for Bacteriophage Isolation
Sewage presents an ideal environment for bacteriophages to thrive, primarily because it contains abundant bacteria, serving as a plentiful food…
Phage Spot Lysis Test: Phage detection technique
What is a Spot Test? A spot test is a simple laboratory method used to determine whether a bacteriophage can…
Agar Overlay (Double-Layer Agar) Technique for Bacteriophage Assays
Overview The agar overlay (double-layer agar) technique is a widely used microbiological method for generating a uniform bacterial lawn on…
Bacteriophages isolation and purification
Bacteriophages, commonly known as phages, are viruses that infect bacteria. The double agar method is used to isolate and purify…
Counting phages: Serial dilution technique
Bacteriophage serial dilution is a reliable and widely used laboratory technique that enables scientists to accurately determine the concentration of…
Techniques to evaluate bacteriophage killing
Bacteriophages are viruses that are particularly reliant on their bacterial hosts' metabolic activity to copy their genetic material and produce…
Phage typing for bacterial strain detection and identification
What is phage typing? Phage typing is a quick and low-cost phenotypic method that uses bacteriophage specificity to detect and…
Isolation of Pseudomonas bacteriophages from fish gastro intestinal tract
Fish plays a vital role in the human diet, and consumption per capita is increasing; however, the expansion of industry…
Isolation of edwardsiella-specific bacteriophages from fish
Aquaculture, the culture of all types of aquatic animals and plants in fresh, brackish, and marine water environments, is one…
Preparation of LB (Luria-Bertani) agar
Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L…
Procedure for Phage isolation
ProcedureCollect crude specimens of approximately 50 g or 100mls of any targeted sample in a sterile sample collection tube (100…
Isolation of Phage DNA – Miniprep Protocol
Extracting the DNA from a bacteriophage is essential in order to study its genetic makeup and identify the genes…
Comprehensive Guide to Bacteriophage Counting through Plaque Assay
Principle of Phage Plaque Assay When a suspension of an infective phage (e.g. T4 phage) is spread over the lawn…