Raphael Hans Lwesya is a PhD researcher and science communicator focused on bacteriophages, microbiology, and bioinformatics. He is the founder of The Phage, a platform dedicated to phage research, news, protocols and job opportunities.
Passing through several waves of the deadly pandemic brought the human race into consideration of doing extensive research on vaccines. It is…
An illustration of ARG transfer by T bacteriophages Because of the rise in antibiotic resistance, effective prevention of pathogens is becoming a…
where do people get phage therapy? Despite the reinstated attention it has gained in recent years, Phage therapy is still limited to…
In the world of booming antimicrobial resistance, scientists worldwide struggle to find a proper method of treating superbugs. Recently, the savior has…
The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the representative standard…
Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add into 300…
Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably Trypticasein…
Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution…
Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further…
Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm, Whatman)
