PHAGE THERAPY
The accessibility of phage therapy services
where do people get phage therapy? Despite the reinstated attention it has gained in recent years, Phage therapy is still limited to…
Author: Raphael Hans Lwesya
Raphael Hans Lwesya is a PhD researcher and science communicator focused on bacteriophages, microbiology, and bioinformatics. He is the founder of The Phage, a platform dedicated to phage research, news, protocols and job opportunities.
Research digest
DendriPep: Innovative macromolecules that stabilize bacteriophages as a medicine
In the world of booming antimicrobial resistance, scientists worldwide struggle to find a proper method of treating superbugs. Recently, the savior has…
PROTOCOL
Plaque-Based Optimization of the Phages Lytic Characteristics
The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the representative standard…
PROTOCOL
Production of the Transient Phage Stock
Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add into 300…
PROTOCOL
Bacterial Stock Culture preparation
Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably Trypticasein…
PROTOCOL
Preparation of anti-phage agent
Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution…
PROTOCOL
Preparation of 13% Pomegranate Rind Extract (PRE)
Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further…
PROTOCOL
Preparation of FeSO4.7H2O Solution
Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm, Whatman)
PROTOCOL
Preparation of SM buffer (lambda diluent)
SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That…
PROTOCOL
Preparation of Phosphate Buffered Saline (PBS)
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a water-based salt solution…