Written by 3:07 pm PREPARATION, PROTOCOL, Reagents

Preparation of anti-phage agent

 

Procedure

Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%). After about 30 s the colour of the mixture changes to greenish then to black. These mixtures of ferrous sulphate and PRE should be protected from light. 
NOTE: The mixture is active for 45 min after preparation.
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