Preparation of 13% Pomegranate Rind Extract (PRE)

 Procedure

  1. Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min.
  2. Centrifuge (20,000xg, 4 °C, 30 min) (A further purification of the pomegranate extract can be achieved by membrane filtration).
  3. Autoclave supernatant (121 °C, 15 min) and cool.
  4. Store at −20 °C.
  5. Prior to use, mix 1.3 ml of stock solution of PRE (25% w/v) with 8.7 ml of Lambda buffer. The final PRE concentration is 13% (see Note 1.4.2).

About the author

Hello there!

I'm Raphael Hans Lwesya. I have a deep interest in phage research and science communication. I strive to simplify complex ideas and present the latest phage-related research in an easy-to-digest format. Thank you for visiting The Phage blog. If you have any questions or suggestions, please feel free to leave a comment or contact me at [email protected].

Leave a Reply

Receive the latest news

Subscribe To Our Weekly Newsletter

Get notified about new articles