Protocol for Mechanical Dissociation and DNA Extraction of sample (Using QIAmp DNA Mini Kit)
This protocol was designed for microbiome analysis in sputum (although other samples can be used...
Bacteriophage host range determination (Culture-Based Approach)
Bacteriophage host range is by definition a repertoire of bacterial species and strains that are able to support the multiplication of the...
Phage Display procedures (Protocol)
In general, there are 5 steps for phage display technology as below: STEP1: Construct phage display library Recombinant DNA technology is...
The multiplicity of infection (MOI) of the phage explained
The Multiplicity of Infection (MOI) is a crucial metric in bacteriophage research and is defined as...
Preparation of Phage Lysates / bacteriophage enumeration (pfu/ml)
Preparation of Phage Lysates Inoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of...
Isolation of Phage DNA – Miniprep Protocol
Extracting the DNA from a bacteriophage is essential in order to study its genetic makeup and identify the genes that encode its various...
Determination of phage adsorption time
The determination of phage adsorption time is a critical step in the study of phages and their...
Sewage sample processing for bacteriophage isolation
As we have discussed earlier bacteriophages are present in the environment (Read about sewage sample collection here), Sewage is one of the...
Bacteriophage enumeration (plaque assay)
Principle of Phage Plaque Assay When a suspension of an infective phage (e.g. T4 phage) is spread over the lawn of susceptible bacterial...
