Preparation of LB (Luria-Bertani) agar
Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how...
Plaque-Based Optimization of the Phages Lytic Characteristics
The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the...
Production of the Transient Phage Stock
Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes....
Procedure for Phage isolation
Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in...
Bacterial Stock Culture preparation
Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably...
Preparation of anti-phage agent
Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%)....
Preparation of 13% Pomegranate Rind Extract (PRE)
Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge...
Preparation of FeSO4.7H2O Solution
Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm,...
Preparation of SM buffer (lambda diluent)
SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it can...
Preparation of Phosphate Buffered Saline (PBS)
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological...
