Phage Spot Lysis Test
Procedure Prepare bacterial lawns of target bacterial isolates or reference bacterial strains...
Procedure for Phage isolation
Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in a sterile sample collection tube (100...
Bacterial Stock Culture preparation
Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably...
Preparation of anti-phage agent
Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly...
Preparation of 13% Pomegranate Rind Extract (PRE)
Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further...
Preparation of FeSO4.7H2O Solution
Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm,...
Preparation of SM buffer (lambda diluent)
SM buffer is primarily used in biology laboratories, and its main function, like any other buffer,...
Preparation of Phosphate Buffered Saline (PBS)
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a...
Common media used in phage work
microbiological Media List of the media used Luria broth (LB): tryptone 10 g l−1 (HiMedia, Mumbai, India), yeast extract5 g l−1...
How to determination of the number of phage particles in a stock (phage titration)
Phage titration (determination of the number of phage particles in stock) is an essential molecular...
