PROTOCOL

Phage Spot Lysis Test

PROTOCOL, Techniques

Procedure  Prepare bacterial lawns of target bacterial isolates or reference bacterial strains...
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Procedure for Phage isolation

PROTOCOL, Techniques • One Comment

  Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in a sterile sample collection tube (100...
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Bacterial Stock Culture preparation

Cultures, PREPARATION, PROTOCOL

Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably...
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Preparation of anti-phage agent

PREPARATION, PROTOCOL, Reagents

  Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly...
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Preparation of 13% Pomegranate Rind Extract (PRE)

PREPARATION, PROTOCOL, Reagents

 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further...
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Preparation of FeSO4.7H2O Solution

PREPARATION, PROTOCOL, Reagents

Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm,...
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Preparation of SM buffer (lambda diluent)

Buffers, PREPARATION, PROTOCOL • One Comment

SM buffer is primarily used in biology laboratories, and its main function, like any other buffer,...
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Preparation of Phosphate Buffered Saline (PBS)

Buffers, PREPARATION, PROTOCOL

Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a...
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Common media used in phage work

Media, PROTOCOL

microbiological Media List of the media used Luria broth (LB): tryptone 10 g l−1 (HiMedia, Mumbai, India), yeast extract5 g l−1...
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How to determination of the number of phage particles in a stock (phage titration)

PROTOCOL

Phage titration (determination of the number of phage particles in stock) is an essential molecular...
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