PROTOCOL
How to determination of the number of phage particles in a stock (phage titration)
Phage titration (determination of the number of phage particles in stock) is an essential molecular biology technique. Most of the time, it…
Category: PROTOCOL
PROTOCOL
Protocol for Mechanical Dissociation and DNA Extraction of sample (Using QIAmp DNA Mini Kit)
This protocol was designed for microbiome analysis in sputum (although other samples can be used with slight modifications) and was adapted and…
PROTOCOL
Bacteriophage host range determination (Culture-Based Approach)
Bacteriophage host range is by definition a repertoire of bacterial species and strains that are able to support the multiplication of the…
PROTOCOL
Phage Display Procedures (Protocol)
In general, there are 5 steps for phage display technology as below: Step 1: Constructing the Phage Display Library Using sophisticated recombinant…
PROTOCOL
The multiplicity of infection (MOI) of the phage explained
The Multiplicity of Infection (MOI) is a crucial metric in bacteriophage research and is defined as the ratio of the number of…
PROTOCOL
Preparation of Phage Lysates / bacteriophage enumeration (pfu/ml)
Bacteriophage enumeration is a crucial laboratory technique used to quantify the abundance of bacteriophages, viruses that infect and replicate within bacteria. This…
PROTOCOL
Determination of phage adsorption time
The determination of phage adsorption time is a critical step in the study of phages and their interactions with bacteria. This information…
Laboratory procedure
Preparation of SM Buffer without gelatin
SM buffer is primarily used in biology laboratories and, like any other buffer, its primary function is to stabilize the pH. It…