PROTOCOL

Preparation of LB (Luria-Bertani) agar

Media, PREPARATION, PROTOCOL

Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how...
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Plaque-Based Optimization of the Phages Lytic Characteristics

PROTOCOL, Techniques

The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the...
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Production of the Transient Phage Stock

PROTOCOL, Techniques

Procedure  Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes....
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Procedure for Phage isolation

PROTOCOL, Techniques • One Comment

  Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in...
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Bacterial Stock Culture preparation

Cultures, PREPARATION, PROTOCOL

Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably...
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Preparation of anti-phage agent

PREPARATION, PROTOCOL, Reagents

  Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%)....
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Preparation of 13% Pomegranate Rind Extract (PRE)

PREPARATION, PROTOCOL, Reagents

 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge...
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Preparation of FeSO4.7H2O Solution

PREPARATION, PROTOCOL, Reagents

Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm,...
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Preparation of SM buffer (lambda diluent)

Buffers, PREPARATION, PROTOCOL • One Comment

SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it can...
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Preparation of Phosphate Buffered Saline (PBS)

Buffers, PREPARATION, PROTOCOL

Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological...
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