Common media used in phage work

microbiological Media

List of the media used

  1. Luria broth (LB): tryptone 10 g l−1 (HiMedia, Mumbai, India), yeast extract5 g l−1 (HiMedia, Mumbai, India), and sodium chloride 10 g l−1 adjust at pH 7.2.
  2. Luria-agar (LA) consisted of the above with the addition of 14 g l−1 agar(HiMedia, Mumbai, India) use for culture maintenance. (click here to read about the preparation of Luria Bertani Agar)
  3. Bacterial dilutions from 18 h LB cultures grown at 37 °C to be carried out in phosphate-buffered saline (Click here for preparation of PBS).
  4. Top layer agar: Prepare LB with Lambda buffer supplemented with 4 g l−1 agar bacteriology.

About the author

Hello there!
I'm Raphael Hans Lwesya, My true passion lies in the world of phage research and science communication. As a diligent phage researcher and an enthusiastic science communicator, I've founded "," a platform dedicated to unraveling the fascinating universe of bacteriophages – viruses that specifically target microbes. My ultimate mission is to bridge the communication gap between the general public and the often intricate world of scientific concepts. I take pride in simplifying complex ideas, breaking them down into easily understandable pieces, and making cutting-edge phage-related research accessible to a wide audience. Thank you for visiting The Phage blog. If you have got any question or suggestion please drop it as a comment or via [email protected]

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