PROTOCOL

Bacteriophages isolation and purification

BACTERIOPHAGES, PROTOCOL

Bacteriophages, commonly known as phages, are viruses that infect bacteria. The double agar method...
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Counting phages: Serial dilution technique

PROTOCOL • One Comment

Bacteriophage serial dilution is a reliable and widely used laboratory technique that enables scientists to accurately determine the...
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Techniques to evaluate bacteriophage killing

PROTOCOL

Bacteriophages are viruses that are particularly reliant on their bacterial hosts’ metabolic activity to copy their genetic material...
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The best way to Isolate bacteriophage DNA

PROTOCOL • 3 Comments

As someone who has done a lot of bacteriophage research, I know how common it is to get host DNA...
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Isolation of Pseudomonas bacteriophages from fish gastro intestinal tract

PROTOCOL, STUDENT'S PERSPECTIVE • 2 Comments

Fish plays a vital role in the human diet, and consumption per capita is increasing; however, the expansion of industry and agriculture...
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Isolation of edwardsiella-specific bacteriophages from fish

PROTOCOL, STUDENT'S PERSPECTIVE • One Comment

Aquaculture, the culture of all types of aquatic animals and plants in fresh, back rush, and marine water environments, is one of the...
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Agar Overlay (double layer agar) technique

PROTOCOL

Preparation of an overlay This method creates a homogeneous lawn of bacteria within a thin layer of...
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Preparation of LB (Luria-Bertani) agar

Media, PREPARATION, PROTOCOL

Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also...
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Plaque-Based Optimization of the Phages Lytic Characteristics

PROTOCOL, Techniques

The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the...
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Production of the Transient Phage Stock

PROTOCOL, Techniques

Procedure  Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile...
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