Bacteriophages isolation and purification
Bacteriophages, commonly known as phages, are viruses that infect bacteria. The double agar method...
Counting phages: Serial dilution technique
Bacteriophage serial dilution is a reliable and widely used laboratory technique that enables scientists to accurately determine the...
Techniques to evaluate bacteriophage killing
Bacteriophages are viruses that are particularly reliant on their bacterial hosts’ metabolic activity to copy their genetic material...
The best way to Isolate bacteriophage DNA
As someone who has done a lot of bacteriophage research, I know how common it is to get host DNA...
Isolation of Pseudomonas bacteriophages from fish gastro intestinal tract
Fish plays a vital role in the human diet, and consumption per capita is increasing; however, the expansion of industry and agriculture...
Isolation of edwardsiella-specific bacteriophages from fish
Aquaculture, the culture of all types of aquatic animals and plants in fresh, back rush, and marine water environments, is one of the...
Agar Overlay (double layer agar) technique
Preparation of an overlay This method creates a homogeneous lawn of bacteria within a thin layer of...
Preparation of LB (Luria-Bertani) agar
Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also...
Plaque-Based Optimization of the Phages Lytic Characteristics
The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the...
Production of the Transient Phage Stock
Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile...
