PROTOCOL

Sewage sample processing for bacteriophage isolation

PROTOCOL • One Comment

In our previously published article focusing on sewage sample collection, we explored the presence...
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Sewage Sample Collection for Bacteriophage Isolation

PROTOCOL, SAMPLE COLLECTION, Techniques

Sewage presents an ideal environment for bacteriophages to thrive, primarily because it contains abundant bacteria, serving as a plentiful...
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Phage Spot Lysis Test: Phage detection technique

PROTOCOL, Techniques

A spot test is a technique used to determine the presence and activity of bacteriophages against specific bacterial strains. It involves...
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Agar Overlay (double layer agar) technique

PROTOCOL

Preparation of an overlay This method creates a homogeneous lawn of bacteria within a thin layer of...
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Bacteriophages isolation and purification

BACTERIOPHAGES, PROTOCOL

Bacteriophages, commonly known as phages, are viruses that infect bacteria. The double agar method is used to isolate and purify phages...
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Counting phages: Serial dilution technique

PROTOCOL • One Comment

Bacteriophage serial dilution is a reliable and widely used laboratory technique that enables scientists to accurately determine the...
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Techniques to evaluate bacteriophage killing

PROTOCOL

Bacteriophages are viruses that are particularly reliant on their bacterial hosts’ metabolic...
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The best way to Isolate bacteriophage DNA

PROTOCOL • 3 Comments

As someone who has done a lot of bacteriophage research, I know how common it is to get host DNA contamination when attempting to isolate...
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Isolation of Pseudomonas bacteriophages from fish gastro intestinal tract

PROTOCOL, STUDENT'S PERSPECTIVE • 2 Comments

Fish plays a vital role in the human diet, and consumption per capita is increasing; however, the expansion of industry and agriculture...
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Isolation of edwardsiella-specific bacteriophages from fish

PROTOCOL, STUDENT'S PERSPECTIVE • One Comment

Aquaculture, the culture of all types of aquatic animals and plants in fresh, back rush, and marine...
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