Find a protocol, step-by-step.

Protocol v1.0 4h

Bacteriophage Isolation from Soil and Sewage Samples

April 15, 2026 • Isolation

Overview This protocol outlines the isolation and purification of bacteriophages from soil or sewage samples using bacterial host strains. Samples are enriched…

Protocol v1.0 3h

One-Step Growth Curve: Phage Latent Period and Burst Size

March 29, 2026 • Bulking • Wet bench

The one-step growth curve experiment determines the phage life cycle duration, including latent period, rise period, and burst size, by limiting infection…

Protocol

Bacteriophage purification by CsCl density gradient

January 4, 2026 • Purification • Wet bench

Overview After PEG precipitation, bacteriophages can be further purified using a caesium chloride (CsCl) density gradient, which separates intact virions from host…

Protocol v1.0

Bacteriophage precipitation using PEG (PEG/NaCl method)

January 4, 2026 • Bulking • Wet bench

Purpose Concentrate bacteriophages from a cleared lysate using PEG (polyethylene glycol) + NaCl, then resuspend as a smaller-volume, higher-titer phage prep. Safety…

Protocol v1.0

Sewage sample processing for bacteriophage isolation

February 12, 2025

Sewage is a rich source of diverse bacteriophages, particularly coliphages that target coliform bacteria. However, its composition can vary significantly depending on…

Protocol

Prophage Induction Using Mitomycin C

January 7, 2024

Prophages are integrated viral genomes that reside within bacterial chromosomes, remaining dormant until triggered by various environmental stimuli. One of the most…

Protocol

Lysogenic to lytic cycle: Bacteriophage Induction

August 18, 2023

Bacteriophages, particularly template phages, have developed sophisticated tactics for breaching bacterial defenses and binding to the bacterial genome. Within their hosts, they…

Protocol

Sewage Sample Collection for Bacteriophage Isolation

June 26, 2023

Sewage presents an ideal environment for bacteriophages to thrive, primarily because it contains abundant bacteria, serving as a plentiful food source for…

Protocol v1.0.1 1h

Phage Spot Lysis Test: Phage detection technique

May 30, 2023 • Isolation • Wet bench

What is a Spot Test? A spot test is a simple laboratory method used to determine whether a bacteriophage can infect and…

Protocol v1.0.2 30 min

Agar Overlay (Double-Layer Agar) Technique for Bacteriophage Assays

May 29, 2023 • Isolation • Wet bench

Overview The agar overlay (double-layer agar) technique is a widely used microbiological method for generating a uniform bacterial lawn on solid media.…

Protocol

Bacteriophages isolation and purification

February 7, 2023

Bacteriophages, commonly known as phages, are viruses that infect bacteria. The double agar method is used to isolate and purify phages from…

Protocol

Counting phages: Serial dilution technique

February 5, 2023

Bacteriophage serial dilution is a reliable and widely used laboratory technique that enables scientists to accurately determine the concentration of bacteriophages (phages)…