Sewage sample processing for bacteriophage isolation
Sewage is a rich source of diverse bacteriophages, particularly coliphages that target coliform bacteria. However, its composition can vary significantly depending on…
Step-by-step methods, workflows, and lab/bioinformatics guides.
Sewage is a rich source of diverse bacteriophages, particularly coliphages that target coliform bacteria. However, its composition can vary significantly depending on…
Prophages are integrated viral genomes that reside within bacterial chromosomes, remaining dormant until triggered by various environmental stimuli. One of the most…
Bacteriophages, particularly template phages, have developed sophisticated tactics for breaching bacterial defenses and binding to the bacterial genome. Within their hosts, they…
Sewage presents an ideal environment for bacteriophages to thrive, primarily because it contains abundant bacteria, serving as a plentiful food source for…
A spot test is a technique used to determine the presence and activity of bacteriophages against specific bacterial strains. It involves spotting…
Preparation of an overlay This method creates a homogeneous lawn of bacteria within a thin layer of agar across the surface of…
Bacteriophages, commonly known as phages, are viruses that infect bacteria. The double agar method is used to isolate and purify phages from…
Bacteriophage serial dilution is a reliable and widely used laboratory technique that enables scientists to accurately determine the concentration of bacteriophages (phages)…
Bacteriophages are viruses that are particularly reliant on their bacterial hosts’ metabolic activity to copy their genetic material and produce the proteins…