Protocol
Bacteriophage purification by CsCl density gradient
Overview After PEG precipitation, bacteriophages can be further purified using a caesium chloride (CsCl) density gradient, which separates intact virions from host…
Latest protocols
18 protocols found.
Protocol
v1.0
Bacteriophage precipitation using PEG (PEG/NaCl method)
Purpose Concentrate bacteriophages from a cleared lysate using PEG (polyethylene glycol) + NaCl, then resuspend as a smaller-volume, higher-titer phage prep. Safety…
Protocol
v1.0
Sewage sample processing for bacteriophage isolation
Sewage is a rich source of diverse bacteriophages, particularly coliphages that target coliform bacteria. However, its composition can vary significantly depending on…
Protocol
Prophage Induction Using Mitomycin C
Prophages are integrated viral genomes that reside within bacterial chromosomes, remaining dormant until triggered by various environmental stimuli. One of the most…
Protocol
Lysogenic to lytic cycle: Bacteriophage Induction
Bacteriophages, particularly template phages, have developed sophisticated tactics for breaching bacterial defenses and binding to the bacterial genome. Within their hosts, they…
Protocol
Sewage Sample Collection for Bacteriophage Isolation
Sewage presents an ideal environment for bacteriophages to thrive, primarily because it contains abundant bacteria, serving as a plentiful food source for…
Protocol
v1.0.1
1h
Phage Spot Lysis Test: Phage detection technique
What is a Spot Test? A spot test is a simple laboratory method used to determine whether a bacteriophage can infect and…
Protocol
v1.0.2
30 min
Agar Overlay (Double-Layer Agar) Technique for Bacteriophage Assays
Overview The agar overlay (double-layer agar) technique is a widely used microbiological method for generating a uniform bacterial lawn on solid media.…
Protocol
Bacteriophages isolation and purification
Bacteriophages, commonly known as phages, are viruses that infect bacteria. The double agar method is used to isolate and purify phages from…
Protocol
Counting phages: Serial dilution technique
Bacteriophage serial dilution is a reliable and widely used laboratory technique that enables scientists to accurately determine the concentration of bacteriophages (phages)…
Protocol
Techniques to evaluate bacteriophage killing
Bacteriophages are viruses that are particularly reliant on their bacterial hosts’ metabolic activity to copy their genetic material and produce the proteins…
Protocol
v1.0.0
Phage typing for bacterial strain detection and identification
What is phage typing? Phage typing is a quick and low-cost phenotypic method that uses bacteriophage specificity to detect and identify single…