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INTERVIEW

What’s Better: Simplicity or Standardization in Bacteriophage Nomenclature?

Starter package

Understanding Enveloped and Non-Enveloped Viruses

NEWS

Discovery: Bacteriophages Turn Bacteria into ‘Zombie’ Cells

PHAGE RESISTANCE

Saccharibacteria: The parasite that protects its host against phage attack

Phage therapy (Bacteriophage therapy)

April 19, 2021

Soon after Alexander Fleming’s 1928 discovery of penicillin and the beginning of Western medicine’s widespread use of antibiotics in the 1940s, Fleming himself warned…

Phage display procedure in depth

Phage Display Procedures (Protocol)

April 11, 2021

In general, there are 5 steps for phage display technology as below: Step 1: Constructing the Phage Display Library Using sophisticated recombinant DNA techniques,…

Bacteriophage on the surface of bacteria UK innovate

The multiplicity of infection (MOI) of the phage explained

April 6, 2021

The Multiplicity of Infection (MOI) is a crucial metric in bacteriophage research and is defined as the ratio of the number of infectious agents…

Preparation of Phage Lysates / bacteriophage enumeration (pfu/ml)

March 24, 2021

Bacteriophage enumeration is a crucial laboratory technique used to quantify the abundance of bacteriophages, viruses that infect and replicate within bacteria. This process typically…

Isolation of Phage DNA – Miniprep Protocol

March 24, 2021

  Extracting the DNA from a bacteriophage is essential in order to study its genetic makeup and identify the genes that encode its various…

Determination of phage adsorption time

March 14, 2021

The determination of phage adsorption time is a critical step in the study of phages and their interactions with bacteria. This information can provide…

preparation of Gelatin solution, 2%

March 8, 2021

Gelatin solution, 2% To prepare a 2% (w/v) solution: Add 2 g of gelatin to a total volume of 100 ml of H2O and…

Preparation of Tris-Cl

March 8, 2021

  Tris base HCl To prepare a 1 M solution, dissolve 121.1 g of Tris base in 800 mL of H2O. Adjust the pH…

Preparation of SM Buffer without gelatin

March 8, 2021

SM buffer is primarily used in biology laboratories and, like any other buffer, its primary function is to stabilize the pH. It can withstand…

Comprehensive Guide to Bacteriophage Counting through Plaque Assay

February 25, 2021

Principle of Phage Plaque Assay When a suspension of an infective phage (e.g. T4 phage) is spread over the lawn of susceptible bacterial cells…

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