Author: Raphael Hans Lwesya

Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also…

The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the representative standard reference bacterial…

Procedure  Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add into 300 µl of…

  Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in a sterile sample collection tube (100 ml). Mix…

Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably Trypticasein soy agar(TSA),…

  Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%). After…

 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further purification of…

Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm, Whatman)

SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it…