The best way to Isolate bacteriophage DNA January 19, 2023 As someone who has conducted bacteriophage research, I understand how common it is to encounter host DNA contamination when isolating a phage genome. This…
Isolation of Pseudomonas bacteriophages from fish gastro intestinal tract October 3, 2022 Fish plays a vital role in the human diet, and consumption per capita is increasing; however, the expansion of industry and agriculture causes contamination…
Isolation of edwardsiella-specific bacteriophages from fish September 11, 2022 Aquaculture, the culture of all types of aquatic animals and plants in fresh, back rush, and marine water environments, is one of the fastest-growing…
Preparation of LB (Luria-Bertani) agar January 1, 2022 Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also…
Plaque-Based Optimization of the Phages Lytic Characteristics December 26, 2021 The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the representative standard reference bacterial…
Production of the Transient Phage Stock December 26, 2021 Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add into 300 µl of…
Procedure for Phage isolation December 26, 2021 Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in a sterile sample collection tube (100 ml). Mix…
Bacterial Stock Culture preparation December 26, 2021 Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably Trypticasein soy agar(TSA),…
Preparation of anti-phage agent December 26, 2021 Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%). After…
Preparation of 13% Pomegranate Rind Extract (PRE) December 26, 2021 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further purification of…
