Preparation of FeSO4.7H2O Solution December 26, 2021 Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm, Whatman)
Preparation of SM buffer (lambda diluent) December 26, 2021 SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it…
Preparation of Phosphate Buffered Saline (PBS) December 26, 2021 Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a water-based salt solution containing disodium…
Common media used in phage work December 26, 2021 microbiological Media List of the media used Luria broth (LB): tryptone 10 g l−1 (HiMedia, Mumbai, India), yeast extract5 g l−1 (HiMedia, Mumbai, India),…
How to determination of the number of phage particles in a stock (phage titration) June 4, 2021 Phage titration (determination of the number of phage particles in stock) is an essential molecular biology technique. Most of the time, it does after…
Protocol for Mechanical Dissociation and DNA Extraction of sample (Using QIAmp DNA Mini Kit) June 2, 2021 This protocol was designed for microbiome analysis in sputum (although other samples can be used with slight modifications) and was adapted and used in…
Bacteriophage host range determination (Culture-Based Approach) May 23, 2021 Bacteriophage host range is by definition a repertoire of bacterial species and strains that are able to support the multiplication of the given virus….
Phage Display Procedures (Protocol) April 11, 2021 In general, there are 5 steps for phage display technology as below: Step 1: Constructing the Phage Display Library Using sophisticated recombinant DNA techniques,…
The multiplicity of infection (MOI) of the phage explained April 6, 2021 The Multiplicity of Infection (MOI) is a crucial metric in bacteriophage research and is defined as the ratio of the number of infectious agents…
Preparation of Phage Lysates / bacteriophage enumeration (pfu/ml) March 24, 2021 Bacteriophage enumeration is a crucial laboratory technique used to quantify the abundance of bacteriophages, viruses that infect and replicate within bacteria. This process typically…
