One-Step Growth Curve: Phage Latent Period and Burst Size
The one-step growth curve experiment determines the phage life cycle duration, including latent period, rise period, and burst size, by limiting infection…
Protocol type: Wet bench
Bacteriophage purification by CsCl density gradient
Overview After PEG precipitation, bacteriophages can be further purified using a caesium chloride (CsCl) density gradient, which separates intact virions from host…
Bacteriophage precipitation using PEG (PEG/NaCl method)
Purpose Concentrate bacteriophages from a cleared lysate using PEG (polyethylene glycol) + NaCl, then resuspend as a smaller-volume, higher-titer phage prep. Safety…
Phage Spot Lysis Test: Phage detection technique
What is a Spot Test? A spot test is a simple laboratory method used to determine whether a bacteriophage can infect and…
Agar Overlay (Double-Layer Agar) Technique for Bacteriophage Assays
Overview The agar overlay (double-layer agar) technique is a widely used microbiological method for generating a uniform bacterial lawn on solid media.…
Isolation of edwardsiella-specific bacteriophages from fish
Aquaculture, the culture of all types of aquatic animals and plants in fresh, brackish, and marine water environments, is one of the…