Raphael Hans Lwesya | The Phage

How to determination of the number of phage particles in a stock (phage titration)

June 4, 2021

Phage titration (determination of the number of phage particles in stock) is an essential molecular biology technique. Most of the time, it does after…

Protocol for Mechanical Dissociation and DNA Extraction of sample (Using QIAmp DNA Mini Kit)

June 2, 2021

This protocol was designed for microbiome analysis in sputum (although other samples can be used with slight modifications) and was adapted and used in…

The virus that lives in sputum to feed on deadly bacteria (in patients with Cystic fibrosis)

June 2, 2021

Introduction: There is a recent study about the extraction and detection of phage DNA from a sputum sample done by scientists from Stanford University…

Bacteriophage host range determination (Culture-Based Approach)

May 23, 2021

Bacteriophage host range is by definition a repertoire of bacterial species and strains that are able to support the multiplication of the given virus….

Is phage therapy a solution to Netherton Syndrome?

May 5, 2021

Netherton Syndrome (NS) is a rare genetic disorder that significantly weakens the skin’s barrier, making patients highly susceptible to chronic infections and allergic reactions….

Phage therapy (Bacteriophage therapy)

April 19, 2021

Soon after Alexander Fleming’s 1928 discovery of penicillin and the beginning of Western medicine’s widespread use of antibiotics in the 1940s, Fleming himself warned…

Phage Display Procedures (Protocol)

April 11, 2021

In general, there are 5 steps for phage display technology as below: Step 1: Constructing the Phage Display Library Using sophisticated recombinant DNA techniques,…

Bacteriophage on the surface of bacteria-UK innovate

The multiplicity of infection (MOI) of the phage explained

April 6, 2021

The Multiplicity of Infection (MOI) is a crucial metric in bacteriophage research and is defined as the ratio of the number of infectious agents…

Preparation of Phage Lysates / bacteriophage enumeration (pfu/ml)

March 24, 2021

Bacteriophage enumeration is a crucial laboratory technique used to quantify the abundance of bacteriophages, viruses that infect and replicate within bacteria. This process typically…

Isolation of Phage DNA – Miniprep Protocol

March 24, 2021

Extracting the DNA from a bacteriophage is essential in order to study its genetic makeup and identify the genes that encode its various…