Procedure
- First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3.
- Sterilized by membrane filtration (0.45 lm, Whatman)
About the Author
Related articles
More stories you might enjoy from The Phage.
PROTOCOL
The best way to Isolate bacteriophage DNA
As someone who has conducted bacteriophage research, I understand how common it is to encounter host DNA contamination when isolating…
PROTOCOL
Plaque-Based Optimization of the Phages Lytic Characteristics
The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the…
PROTOCOL
Production of the Transient Phage Stock
Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add…
Leave a Reply
You must be logged in to post a comment.