Sterilization by filtration method
In microbiology, it is imperative to have sterile solutions in order to prevent contamination in...
Plaque-Based Optimization of the Phages Lytic Characteristics
The isolates of wild lytic phages from the transient stocks are propagated with the corresponding host bacterial isolates and the...
Production of the Transient Phage Stock
Procedure Cut 5–10 plaques with a sterile Pasteur pipette and place them in 1.5 ml sterile Eppendorf micro-centrifuge tubes. Add...
Phage Spot Lysis Test
Procedure Prepare bacterial lawns of target bacterial isolates or reference bacterial strains...
Procedure for Phage isolation
Procedure Collect crude specimens of approximately 50 g or 100mls of any targeted sample in a sterile sample collection tube (100...
