How is a phage cocktail prepared?
Phage cocktails are an innovative solution for treating bacterial infections. They are a mixture of...
Preparation of SM Buffer with gelatin (lambda diluent)
SM buffer with gelatin is a commonly used buffer solution for various cellular and biochemical applications. It is a versatile...
Preparation of LB (Luria-Bertani) agar
Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also...
Bacterial Stock Culture preparation
Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained...
Preparation of anti-phage agent
Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%)....
Preparation of 13% Pomegranate Rind Extract (PRE)
Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A...
Preparation of FeSO4.7H2O Solution
Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3....
Preparation of SM buffer (lambda diluent)
SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it can...
Preparation of Phosphate Buffered Saline (PBS)
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a...
How to make an outstanding media (agar) plate
Whether they contain TSA, MHA, LB, M9, or any other medium, making agar plates is a simple...
