How is a phage cocktail prepared? February 6, 2023 Phage cocktails are an innovative solution for treating bacterial infections. They are a mixture of two or more purified bacteriophages, which are viruses that
Preparation of SM Buffer with gelatin (lambda diluent) February 5, 2023 SM buffer with gelatin is a commonly used buffer solution for various cellular and biochemical applications. It is a versatile buffer that can
Preparation of LB (Luria-Bertani) agar January 1, 2022 Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also
Bacterial Stock Culture preparation December 26, 2021 Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained on any general media preferably Trypticasein soy agar(TSA),
Preparation of anti-phage agent December 26, 2021 Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%). After
Preparation of 13% Pomegranate Rind Extract (PRE) December 26, 2021 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A further purification of
Preparation of FeSO4.7H2O Solution December 26, 2021 Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3. Sterilized by membrane filtration (0.45 lm, Whatman)
Preparation of SM buffer (lambda diluent) December 26, 2021 SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it
Preparation of Phosphate Buffered Saline (PBS) December 26, 2021 Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a water-based salt solution containing disodium
How to make an outstanding media (agar) plate June 5, 2021 Whether they contain TSA, MHA, LB, M9, or any other medium, making agar plates is a simple procedure. But there are a few finer