PREPARATION

How is a phage cocktail prepared?

AMR, PHAGE RESISTANCE, PHAGE TECHNOLOGY, PREPARATION • 2 Comments

Phage cocktails are an innovative solution for treating bacterial infections. They are a mixture of...
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Preparation of SM Buffer with gelatin (lambda diluent)

PREPARATION • One Comment

  SM buffer with gelatin is a commonly used buffer solution for various cellular and biochemical applications. It is a versatile...
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Preparation of LB (Luria-Bertani) agar

Media, PREPARATION, PROTOCOL

Luria-Bertani is the most preferred media to be used in phage research. This protocol describes how to make 1 L of Luria-Bertani agar (also...
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Bacterial Stock Culture preparation

Cultures, PREPARATION, PROTOCOL

Procedure Prior to the procedure, a stock culture of the phage target bacteria should be maintained...
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Preparation of anti-phage agent

PREPARATION, PROTOCOL, Reagents

  Procedure Immediately prior to use, transfer 3.3 ml of 13% PRE added to 7 ml of freshly prepared ferrous sulphate solution (0.01%)....
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Preparation of 13% Pomegranate Rind Extract (PRE)

PREPARATION, PROTOCOL, Reagents

 Procedure Blend pomegranate rind in distilled water (25% w/v) and boil for 10 min. Centrifuge (20,000xg, 4 °C, 30 min) (A...
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Preparation of FeSO4.7H2O Solution

PREPARATION, PROTOCOL, Reagents

Procedure First freshly prepare a solution (0.01%) of FeSO4.7H2O in Lambda buffer, pH 6.3....
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Preparation of SM buffer (lambda diluent)

Buffers, PREPARATION, PROTOCOL • One Comment

SM buffer is primarily used in biology laboratories, and its main function, like any other buffer, is to stabilize the pH. That is, it can...
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Preparation of Phosphate Buffered Saline (PBS)

Buffers, PREPARATION, PROTOCOL

Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research including phage work. It is a...
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How to make an outstanding media (agar) plate

PREPARATION

Whether they contain TSA, MHA, LB, M9, or any other medium, making agar plates is a simple...
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