Which kind of lab is supposed to handle bacteriophages?

Bacteriophages are viruses that affect bacteria and not plants, human or any other mammals, Due to their specificity, there fore it pose no harm to other creatures except that specific bacteria host. To determine whether which laboratory can handle which bacteriophage it depends on the host used instead of the phage handled, there fore the determinant is bacteria and not the virus. For example bacteriophages for Mycobacterium must be handled in higher labs due to the fact that at one point the host (which is the deadly bacteria) will be used.  Lets polish our knowledge on bio-safety laboratory levels.

 Bio-safety Level 1

Biosafety level one, the lowest level, applies to work with agents that usually pose a minimal potential threat to laboratory workers and the environment and do not consistently cause disease in healthy adults. Research with these agents is generally performed on standard open laboratory benches without the use of special containment equipment. BSL 1 labs are not usually isolated from the general building. Training on the specific procedures is given to the lab personnel, who are supervised by a trained microbiologist or scientist.

Standard microbiology practices are usually enough to protect laboratory workers and other employees in the building. These include mechanical pipetting only (no mouth pipetting allowed), safe sharps handling, avoidance of splashes or aerosols, and decontamination of all work surfaces when work is complete, e.g., daily. Decontamination of spills is done immediately, and all potentially infectious materials are decontaminated prior to disposal, generally by autoclaving. Standard microbiological practices also require attention to personal hygiene, i.e., hand washing and a prohibition on eating, drinking or smoking in the lab. Normal laboratory personal protective equipment is generally worn, consisting of eye protection, gloves and a lab coat or gown. Biohazard signs are posted and access to the lab is limited whenever infectious agents are present.

Bio-safety Level 2

Biosafety level two would cover work with agents associated with human disease, in other words, pathogenic or infectious organisms posing a moderate hazard. Examples are the equine encephalitis viruses and HIV when performing routine diagnostic procedures or work with clinical specimens. Therefore, because of their potential to cause human disease, great care is used to prevent percutaneous injury (needlesticks, cuts and other breaches of the skin), ingestion and mucous membrane exposures in addition to the standard microbiological practices of BSL 1. Contaminated sharps are handled with extreme caution. Use of disposable syringe-needle units and appropriate puncture-resistant sharps containers is mandatory. Direct handling of broken glassware is prohibited, and decontamination of all sharps prior to disposal is standard practice. The laboratory’s written biosafety manual details any needed immunizations (e.g., hepatitis B vaccine or TB skin testing) and whether serum banking is required for at-risk lab personnel. Access to the lab is more controlled than for BSL 1 facilities. Immunocompromised, immunosuppressed and other persons with increased risk for infection may be denied admittance at the discretion of the laboratory director.

BSL 2 labs must also provide the next level of barriers, i.e., specialty safety equipment and facilities. Preferably, this is a Class II biosafety cabinet or equivalent containment device for work with agents and an autoclave or other suitable method for decontamination within the lab. A readily available eyewash station is needed. Selfclosing lockable doors and biohazard warning signs are also required at all access points.

Bio-safety Level 3

Yellow fever, St. Louis encephalitis and West Nile virus are examples of agents requiring biosafety level 3 practices and containment. Work with these agents is strictly controlled and must be registered with all appropriate government agencies.2 These are indigenous or exotic agents that may cause serious or lethal disease via aerosol transmission, i.e., simple inhalation of particles or droplets. The pathogenicity and communicability of these agents dictates the next level of protective procedures and barriers. Add to all the BSL 2 practices and equipment even more stringent access control and decontamination of all wastes, including lab clothing before laundering, within the lab facility. Baseline serum samples are collected from all lab and other at-risk personnel as appropriate.

More protective primary barriers are used in BSL 3 laboratories, including solid-front wraparound gowns, scrub suits or coveralls made of materials such as Tyvek® and respirators as necessary. Facility design should incorporate self-closing double-door access separated from general building corridors. The ventilation must provide ducted, directional airflow by drawing air into the lab from clean areas and with no recirculation.

Bio-safety Level 4

Agents requiring BSL 4 facilities and practices are extremely dangerous and pose a high risk of life-threatening disease. Examples are the Ebola virus, the Lassa virus, and any agent with unknown risks of pathogenicity and transmission (Laboratory insider listed top ten of the most lethal viruses here). These facilities provide the maximum protection and containment. To the BSL 3 practices, we add requirements for complete clothing change before entry, a shower on exit and decontamination of all materials prior to leaving the facility.

The BSL 4 laboratory should contain a Class III biological safety cabinet but may use a Class I or II BSC in combination with a positive-pressure, air-supplied full-body suit. Usually, BSL 4 laboratories are in separate buildings or a totally isolated zone with dedicated supply and exhaust ventilation. Exhaust streams are filtered through high-efficiency particulate air (HEPA) filters, depending on the agents used.

Biosaftety lab levels infographic by CDC
Biosafety lab levels by CDC


Raphael Hans

Young scientist, phage enthusiast, and passionate about driving the development of bacteriophage therapy and application. Working as a research assistant at Makerere University.

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