Preparation of SM Buffer without gelatin

 

ReagentAmount  Final concentration
NaCl5.8 g100 mM
MgSO4•7H2O2 g8 mM
Tris-Cl (1 M, pH 7.5)50 ml50 mM
H2Oto 1 liter
To prepare 1 liter of SM buffer, dissolve the NaCl and MgSO4•7H2O in 800 ml of H2O; add the Tris-Cl; and adjust the volume to 1 liter with H2O. Sterilize the buffer by autoclaving for 20 minutes at 15 psi (1.05 kg/cm2) on liquid cycle. After the solution has cooled, dispense 50-ml aliquots into sterile containers. SM buffer may be stored indefinitely at room temperature. Discard each aliquot after use to minimize the chance of contamination. SM buffer may also be prepared with 0.002% (w/v) gelatin; see the recipe for SM buffer with gelatin. To prepare SM Buffer with gelatin click here and to prepare Tris-cl click here


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